Analysis of observed S protein RBD mutations

Relevant link: Mutation Tracker for genome-wide analysis

Mutation	Worldwide observed		BFE change (kcal/mol)*		Antibody disruption
Mutation	Counts	Rank	Value	Rank	Counts^♦	Ratio(%)^♠	Rank
T478K	2053531	1	0.9994	3	2	1.54	714
L452R	1990939	2	0.5752	39	39	30.0	143
N501Y	936596	3	0.5499	42	24	18.46	235
S477N	126383	4	0.0180	493	0	0.0	800
E484K	125704	5	0.0946	327	38	29.23	146
K417N	102746	6	0.1661	209	53	40.77	96
E484A	91531	7	-0.0183	552	42	32.31	127
G339D	90853	8	0.1117	292	5	3.85	552
S373P	90703	9	0.0835	352	7	5.38	457
Q493R	90606	10	0.1271	267	30	23.08	195
S375F	90589	11	0.0609	399	1	0.77	782
Y505H	90504	12	-0.2060	698	43	33.08	123
Q498R	90459	13	-0.0969	651	24	18.46	231
S371F	89534	14	0.1989	173	0	0.0	795
N440K	87781	15	0.6161	32	0	0.0	815
D405N	87209	16	0.2673	128	6	4.62	513
T376A	87110	17	-0.0016	533	10	7.69	391
R408S	86851	18	0.1010	313	42	32.31	126
K417T	67533	19	0.0116	504	37	28.46	152
N439K	19531	20	0.1792	191	11	8.46	371
E484Q	8852	21	0.0057	513	30	23.08	193
R346K	7814	22	0.1234	274	6	4.62	507
F490S	6928	23	0.4406	68	51	39.23	105
S494P	6225	24	0.0902	338	62	47.69	78
G446V	5514	25	0.1583	219	9	6.92	422
A520S	5080	26	0.1495	237	3	2.31	618
L452Q	3970	27	0.9802	5	27	20.77	213
G446S	3848	28	0.0046	519	5	3.85	548
G496S	3580	29	0.0187	491	24	18.46	236
S477I	3565	30	-0.0002	530	2	1.54	693
S371P	3281	31	0.0872	347	19	14.62	276
A522S	3251	32	0.1283	264	2	1.54	695
A522V	2825	33	0.0705	379	1	0.77	767
Q414R	2749	34	0.0708	377	7	5.38	451
N501T	2681	35	0.4514	63	17	13.08	299
P479S	2610	36	0.3844	87	3	2.31	619
A411S	2401	37	0.5023	55	11	8.46	378
R357K	2307	38	0.1393	250	5	3.85	547
V367L	1964	39	0.0465	427	0	0.0	818
P384L	1919	40	0.2681	126	18	13.85	280
T385I	1883	41	0.0314	470	2	1.54	698
V367F	1737	42	0.1764	193	0	0.0	840
A475V	1647	43	0.3069	115	10	7.69	390
V483F	1631	44	0.5428	44	5	3.85	544
Q414K	1556	45	0.1234	275	3	2.31	621
S459F	1457	46	0.4998	56	0	0.0	842
R408I	1448	47	0.1949	174	25	19.23	227
L455F	1436	48	-0.0415	581	20	15.38	269
N440S	1389	49	0.1499	235	2	1.54	700
D427N	1376	50	-0.1133	659	1	0.77	762
R346S	1327	51	0.0374	455	20	15.38	264
N354K	1261	52	0.1993	172	2	1.54	701
A348S	1229	53	0.4616	62	3	2.31	622
Y449S	1212	54	-0.8112	772	85	65.38	22
V382L	1185	55	0.0355	459	2	1.54	703
F490L	1055	56	0.5139	50	33	25.38	180
L452M	1053	57	0.5966	35	7	5.38	445
S494L	1030	58	0.0263	476	38	29.23	148
T376I	998	59	0.1338	257	7	5.38	444
A344S	976	60	0.1605	215	3	2.31	630
A352S	960	61	0.2576	131	6	4.62	505
S477R	940	62	0.0820	356	0	0.0	850
K356R	850	63	0.0412	444	4	3.08	614
G476S	826	64	0.1566	225	10	7.69	392
A419S	751	65	0.4147	78	4	3.08	613
N450K	704	66	0.3535	94	10	7.69	389
E471Q	696	67	0.5230	48	2	1.54	705
D427V	684	68	0.1294	262	1	0.77	744
P384S	672	69	0.1151	288	27	20.77	210
T470N	657	70	-0.0310	568	10	7.69	385
P463S	604	71	0.1144	290	4	3.08	612
T470I	595	72	0.0208	485	2	1.54	708
P479L	592	73	0.2009	170	2	1.54	709
R346I	581	74	0.0763	370	15	11.54	327
S514F	580	75	0.3519	95	2	1.54	710
Y508H	566	76	0.7562	19	5	3.85	536
K444N	564	77	0.1024	310	17	13.08	293
F338L	545	78	0.0239	479	5	3.85	534
E516Q	525	79	0.0608	400	1	0.77	753
Q414H	525	80	-0.0681	625	2	1.54	684
T478I	515	81	0.1269	269	2	1.54	680
N354T	505	82	0.1680	206	3	2.31	626
A522P	504	83	0.0901	339	2	1.54	671
V362F	494	84	0.1082	300	1	0.77	790
K444R	493	85	0.2006	171	10	7.69	403
T385N	490	86	0.0049	516	6	4.62	490
S477G	482	87	-0.0234	556	6	4.62	492
S373L	472	88	0.0220	484	0	0.0	835
I434V	451	89	0.1286	263	7	5.38	467
N460S	400	90	0.1558	226	9	6.92	413
P499R	389	91	0.0440	437	13	10.0	355
Y453F	383	92	0.5225	49	4	3.08	605
V503F	362	93	0.1639	212	3	2.31	628
N370S	359	94	-0.0569	607	4	3.08	601
Q493E	359	95	-0.0346	573	40	30.77	138
D427G	353	96	-0.0433	583	4	3.08	599
V433I	344	97	0.1571	221	18	13.85	278
T478R	329	98	-0.0100	541	1	0.77	785
R403K	327	99	0.1778	192	42	32.31	131
Y449H	318	100	-0.4881	745	21	16.15	259
V503I	307	101	0.1303	260	0	0.0	825
T345S	305	102	-0.0787	638	7	5.38	475
A475S	303	103	-0.0732	634	33	25.38	182
V483I	283	104	0.6702	25	1	0.77	780
V401L	279	105	0.7544	20	41	31.54	133
N501S	272	106	0.1672	207	26	20.0	218
F456L	269	107	-0.0822	640	89	68.46	16
A435S	267	108	0.1390	251	8	6.15	432
V367I	264	109	0.0317	468	0	0.0	830
L335F	262	110	0.0604	402	0	0.0	831
G339S	261	111	0.0559	409	1	0.77	739
S459Y	255	112	0.5234	47	0	0.0	833
R346G	253	113	-0.0462	591	16	12.31	312
K458N	245	114	0.0032	523	8	6.15	442
D427Y	243	115	0.1732	198	2	1.54	672
T430I	243	116	0.0684	384	4	3.08	594
I410V	235	117	0.7105	22	27	20.77	211
V483A	232	118	0.6695	26	17	13.08	291
L517F	231	119	0.2432	141	0	0.0	828
A372V	230	120	-0.0479	593	1	0.77	792
I468V	224	121	0.0409	445	6	4.62	511
S359N	221	122	0.0615	398	3	2.31	629
S349P	219	123	0.0888	342	57	43.85	89
S514T	214	124	0.1711	204	2	1.54	676
T415N	213	125	0.0374	453	1	0.77	747
V367A	212	126	-0.0654	620	0	0.0	846
A352V	211	127	0.0848	349	4	3.08	592
N354D	209	128	0.1248	273	10	7.69	388
P479R	201	129	-0.0461	590	2	1.54	675
A520V	199	130	0.0995	315	0	0.0	804
V407I	198	131	0.2353	147	18	13.85	288
Y449N	198	132	-0.5420	750	40	30.77	140
Q474H	196	133	0.0799	360	2	1.54	674
K417R	196	134	0.0105	507	35	26.92	170
E406Q	195	135	0.1717	202	17	13.08	303
E340K	192	136	-0.0048	535	2	1.54	673
A348T	187	137	0.4633	60	3	2.31	625
S359T	183	138	0.0243	477	1	0.77	745
P426S	180	139	0.1212	278	6	4.62	503
P521S	175	140	0.1069	303	2	1.54	707
F464L	174	141	0.0378	452	9	6.92	415
N354S	168	142	0.1455	244	6	4.62	512
F486L	167	143	0.1456	243	52	40.0	100
R408G	157	144	-0.0141	548	57	43.85	88
K378N	155	145	0.0573	407	25	19.23	228
V445A	153	146	0.0961	323	8	6.15	435
A344T	153	147	0.1201	281	3	2.31	624
E484G	149	148	0.0398	447	35	26.92	168
V445F	142	149	0.2530	133	3	2.31	623
N481K	140	150	0.1084	299	1	0.77	748
S359R	140	151	0.0658	391	0	0.0	817
V445I	138	152	0.3273	107	0	0.0	847
P337L	138	153	0.0374	454	4	3.08	575
L441R	137	154	-0.0288	565	8	6.15	430
S371T	137	155	0.0802	359	2	1.54	736
P499S	136	156	-0.0874	644	14	10.77	337
N440Y	136	157	0.1842	185	4	3.08	585
I402V	130	158	0.4131	80	23	17.69	242
P337S	130	159	0.1039	308	4	3.08	580
D427H	126	160	-0.0375	576	1	0.77	786
K356N	124	161	-0.1484	677	9	6.92	418
E484D	124	162	0.1727	200	41	31.54	135
G504S	124	163	0.0301	472	13	10.0	341
F490V	124	164	0.6497	28	40	30.77	137
R408K	123	165	0.0491	420	14	10.77	333
L513F	123	166	0.0386	451	0	0.0	821
G446R	121	167	0.3295	105	7	5.38	447
K444M	120	168	0.4243	75	12	9.23	360
E340D	119	169	0.0162	498	4	3.08	570
G413R	117	170	0.1567	224	5	3.85	529
V401I	116	171	0.2891	116	62	47.69	73
A411V	116	172	0.4440	67	12	9.23	369
V341I	113	173	0.0630	396	1	0.77	752
E484V	109	174	-0.3604	729	21	16.15	258
G485S	108	175	0.1359	255	10	7.69	397
Q493L	108	176	0.2279	149	52	40.0	102
D405Y	108	177	0.1231	276	6	4.62	479
P491S	107	178	0.3992	84	10	7.69	394
G485V	105	179	-0.0570	608	22	16.92	249
D428E	105	180	-0.0401	579	3	2.31	617
L518I	103	181	0.0056	514	4	3.08	582
R346T	102	182	0.0530	414	17	13.08	301
Q493K	102	183	0.4321	71	26	20.0	222
G482V	101	184	0.0707	378	7	5.38	473
V483L	98	185	0.4729	58	3	2.31	616
P499L	98	186	-0.0387	577	7	5.38	466
N394S	98	187	0.0530	415	2	1.54	726
L441F	97	188	0.0632	395	1	0.77	755
E471G	97	189	0.0450	432	6	4.62	510
E516A	96	190	-0.0481	595	5	3.85	523
D427A	91	191	-0.0844	641	1	0.77	759
T376N	90	192	0.0640	394	11	8.46	370
E340A	89	193	-0.0634	614	5	3.85	521
N354H	89	194	0.0912	334	0	0.0	845
P479H	88	195	-0.0006	531	1	0.77	763
L517I	88	196	0.0366	456	0	0.0	844
A363V	85	197	0.1884	182	3	2.31	627
I434M	85	198	0.1832	188	7	5.38	465
K356E	83	199	0.0508	417	7	5.38	460
S359G	82	200	0.0796	361	5	3.85	526
A372P	82	201	0.1384	253	16	12.31	310
K458R	82	202	0.0928	330	9	6.92	407
E465D	81	203	0.4285	72	4	3.08	590
D428G	81	204	-0.0430	582	6	4.62	498
N460Y	81	205	0.1683	205	9	6.92	416
T376S	81	206	-0.0269	562	9	6.92	411
N370K	81	207	0.0961	324	2	1.54	730
A397V	79	208	0.0993	316	2	1.54	731
T333I	78	209	0.0597	403	0	0.0	814
L390F	77	210	0.0442	436	0	0.0	799
P507S	77	211	-0.1047	658	78	60.0	42
A344V	76	212	-0.0402	580	1	0.77	789
F374L	75	213	-0.1762	689	7	5.38	456
G482S	75	214	0.2845	117	3	2.31	620
Y451H	75	215	0.1492	238	12	9.23	357
F490Y	73	216	0.4146	79	11	8.46	375
Q493H	73	217	0.2385	145	23	17.69	243
N450D	72	218	0.2105	162	18	13.85	282
G485A	72	219	0.0300	474	11	8.46	380
G485R	70	220	0.0672	389	39	30.0	145
N501I	68	221	0.3833	88	29	22.31	200
N460I	68	222	0.1907	180	13	10.0	342
G485D	68	223	0.2440	139	24	18.46	232
P337T	68	224	0.0129	502	4	3.08	589
E340V	67	225	-0.1317	667	5	3.85	524
I358V	67	226	-0.0968	650	3	2.31	632
G413V	66	227	0.1373	254	5	3.85	518
G504D	65	228	0.1103	296	43	33.08	125
Q414L	64	229	-0.0593	611	7	5.38	470
S494A	64	230	0.1729	199	26	20.0	221
I472V	63	231	-0.1265	664	3	2.31	635
L368I	63	232	0.1205	280	3	2.31	666
P521L	62	233	0.0877	345	2	1.54	735
H519Y	62	234	0.0233	481	2	1.54	734
F486I	62	235	0.2697	125	43	33.08	124
P499H	61	236	0.1470	239	13	10.0	345
T500S	59	237	-0.1363	673	8	6.15	431
A411T	59	238	0.2709	124	13	10.0	350
L441I	59	239	-0.0239	558	2	1.54	733
K356Q	59	240	-0.0637	616	5	3.85	522
A348V	58	241	0.2680	127	9	6.92	423
N448S	58	242	-0.5125	747	12	9.23	363
K378R	58	243	0.0881	344	19	14.62	274
E465G	57	244	0.2747	120	6	4.62	481
D405G	56	245	0.4687	59	20	15.38	262
N388D	56	246	0.2499	136	4	3.08	581
N460D	56	247	0.0300	473	28	21.54	207
N440T	55	248	0.2791	119	3	2.31	656
T415A	55	249	-0.0142	549	0	0.0	811
H519L	55	250	-0.0678	624	3	2.31	667
E471D	54	251	0.4396	69	2	1.54	732
I468T	54	252	0.0727	374	6	4.62	483
G482D	53	253	0.3401	98	5	3.85	520
V511I	53	254	0.1187	285	6	4.62	496
S477T	51	255	-0.0539	601	1	0.77	791
H519Q	51	256	-0.0041	534	0	0.0	816
G446D	51	257	0.0401	446	15	11.54	323
E406D	50	258	0.3114	112	13	10.0	348
Y351F	49	259	0.4216	76	4	3.08	572
T523I	49	260	0.0357	458	2	1.54	729
N481S	49	261	0.0862	348	2	1.54	728
G447V	48	262	-0.5607	753	17	13.08	295
K378M	47	263	0.1851	184	21	16.15	257
G446A	46	264	-0.0672	623	7	5.38	459
P521R	46	265	0.0827	354	0	0.0	801
Y421F	46	266	0.9395	8	12	9.23	358
V362L	45	267	0.0964	321	1	0.77	768
F342L	45	268	-0.2795	715	8	6.15	433
A419T	45	269	0.2388	144	11	8.46	372
A475T	44	270	0.0513	416	20	15.38	261
K444T	44	271	0.5999	34	13	10.0	349
A520T	43	272	0.1340	256	1	0.77	769
S469A	43	273	0.0839	351	1	0.77	770
I418V	43	274	0.4258	73	36	27.69	165
G413W	43	275	0.1582	220	15	11.54	328
N370H	43	276	0.0187	492	1	0.77	751
G339V	43	277	0.0332	464	2	1.54	727
S514Y	43	278	0.2269	151	6	4.62	484
I472T	43	279	0.0646	393	18	13.85	283
N440I	42	280	0.3675	89	3	2.31	653
V512I	42	281	0.7336	21	15	11.54	320
Y489H	41	282	-0.1287	665	67	51.54	69
N501H	41	283	0.7617	18	13	10.0	346
S375A	40	284	-0.0240	559	5	3.85	519
S375Y	40	285	0.0786	364	3	2.31	637
D389G	40	286	0.0417	442	1	0.77	760
G476A	40	287	-0.0986	653	12	9.23	361
N334K	40	288	-0.0220	555	0	0.0	848
G496V	38	289	-0.2149	704	42	32.31	129
T523N	38	290	0.0169	497	3	2.31	640
P499T	37	291	0.0494	418	7	5.38	463
S494T	37	292	0.0738	373	16	12.31	315
E471V	37	293	0.1949	175	2	1.54	725
Q498H	36	294	0.2271	150	13	10.0	344
T478A	36	295	-0.0788	639	3	2.31	642
T430S	36	296	0.1005	314	4	3.08	595
V503A	36	297	0.0819	357	7	5.38	469
A520P	35	298	0.1752	195	11	8.46	381
T415S	35	299	0.1324	258	5	3.85	527
A372S	35	300	-0.0705	628	1	0.77	743
G381R	34	301	0.1070	302	11	8.46	376
D420N	34	302	-0.0119	545	6	4.62	514
E340G	34	303	-0.0992	654	6	4.62	495
N481D	34	304	0.0674	388	2	1.54	724
S359I	33	305	0.0792	362	4	3.08	577
Y473F	33	306	0.5299	46	8	6.15	429
A435T	33	307	0.1933	179	7	5.38	453
I434T	33	308	0.3370	100	28	21.54	208
G482R	32	309	0.3388	99	3	2.31	646
V510I	32	310	0.0914	332	10	7.69	396
Y369H	32	311	0.0047	518	9	6.92	419
N448D	32	312	0.4244	74	37	28.46	155
D428Y	32	313	0.2025	169	2	1.54	737
N460K	31	314	0.2741	121	1	0.77	740
T393I	31	315	0.1156	287	3	2.31	648
G339C	31	316	0.0848	350	2	1.54	723
R466I	31	317	0.1324	259	5	3.85	525
V510L	30	318	0.0431	440	8	6.15	427
S375P	30	319	0.0206	487	12	9.23	365
G476D	30	320	-0.1739	688	14	10.77	334
V362I	30	321	0.1148	289	1	0.77	750
K462I	30	322	0.2086	164	6	4.62	494
K462Q	30	323	0.2145	155	4	3.08	602
S371A	29	324	-0.1491	679	2	1.54	722
Y473H	29	325	0.0592	405	15	11.54	322
G504V	29	326	-0.6366	761	22	16.92	250
Y396F	29	327	0.0683	385	3	2.31	651
P479A	29	328	0.5559	41	2	1.54	721
Y380F	28	329	0.0921	331	3	2.31	654
S373A	28	330	-0.1697	686	1	0.77	771
L492V	28	331	0.8225	14	17	13.08	296
E516K	28	332	0.1103	297	2	1.54	720
V395F	28	333	0.1113	293	5	3.85	535
F464S	28	334	-0.0538	600	69	53.08	59
P337H	28	335	0.0741	372	5	3.85	567
Y449D	28	336	-1.0226	785	91	70.0	15
T470A	27	337	-0.0636	615	7	5.38	455
K417M	27	338	0.0000	529	33	25.38	181
F377L	27	339	-0.0171	551	20	15.38	265
E471A	27	340	0.0777	366	2	1.54	719
T523A	26	341	-0.0111	542	0	0.0	837
F456V	26	342	-0.3455	725	62	47.69	77
K356M	26	343	0.0170	496	7	5.38	448
L335V	26	344	-0.0331	571	2	1.54	718
A352T	26	345	0.1544	228	8	6.15	434
D389E	26	346	0.0607	401	0	0.0	824
F486S	26	347	0.5575	40	54	41.54	93
P384H	26	348	0.2082	166	22	16.92	247
G447S	25	349	-0.6370	762	10	7.69	404
F490I	25	350	0.4078	82	39	30.0	144
V503L	25	351	0.4881	57	1	0.77	788
N448T	24	352	-0.0081	539	10	7.69	405
N334Y	24	353	0.1108	294	1	0.77	774
L513I	24	354	0.0129	503	5	3.85	563
Y369C	23	355	0.0177	494	23	17.69	240
S443A	23	356	0.3417	96	3	2.31	645
Y351H	23	357	0.0699	380	5	3.85	564
P384A	23	358	0.0977	318	15	11.54	331
N394D	23	359	0.0444	435	6	4.62	478
S375T	23	360	0.0392	449	5	3.85	566
L517H	23	361	0.0767	368	3	2.31	644
A522T	22	362	0.1168	286	1	0.77	784
E340Q	22	363	-0.0645	617	4	3.08	583
P521T	22	364	0.0876	346	1	0.77	787
A419V	22	365	0.5806	36	19	14.62	275
N481I	22	366	-0.0627	613	2	1.54	717
K458E	22	367	0.2108	160	17	13.08	297
Q493P	21	368	0.0444	434	118	90.77	7
H519P	21	369	0.0647	392	4	3.08	579
D389N	21	370	-0.0155	550	0	0.0	829
D442V	21	371	-0.3597	728	25	19.23	230
N440D	21	372	0.3198	111	6	4.62	497
N370D	21	373	-0.0252	560	5	3.85	565
S371Y	21	374	0.2516	134	0	0.0	834
Y369F	20	375	0.0686	382	2	1.54	716
G413E	20	376	-0.0541	602	18	13.85	284
Q474E	20	377	0.3659	90	8	6.15	441
L335S	20	378	0.0239	480	3	2.31	665
N481Y	20	379	0.0351	460	1	0.77	772
S443F	20	380	-0.9240	779	66	50.77	70
D442Y	20	381	-1.1123	791	50	38.46	107
V511L	19	382	-0.0553	605	10	7.69	386
Y396H	19	383	-0.0333	572	2	1.54	738
A372T	19	384	-0.0597	612	0	0.0	839
W436L	19	385	-0.2617	713	16	12.31	316
N354Y	19	386	0.1042	307	4	3.08	569
R408T	19	387	-0.0571	609	48	36.92	109
D405E	19	388	0.2476	137	11	8.46	379
A348E	18	389	0.5401	45	14	10.77	336
R457S	18	390	0.3281	106	52	40.0	103
A397S	18	391	0.0483	423	9	6.92	414
C361S	18	392	0.0692	381	6	4.62	504
I472L	18	393	-0.0485	596	4	3.08	588
R403I	18	394	-0.6344	760	71	54.62	55
D427E	17	395	-0.0692	627	1	0.77	764
L455S	17	396	-0.2100	701	39	30.0	142
D420Y	17	397	0.5024	54	21	16.15	255
Q474R	17	398	0.0331	465	5	3.85	561
H519D	16	399	-0.0318	570	5	3.85	562
P521Q	16	400	0.0908	335	1	0.77	756
I472F	16	401	0.5490	43	18	13.85	285
K417E	16	402	0.0115	505	43	33.08	122
S366F	16	403	0.2242	153	4	3.08	611
V395I	15	404	0.0573	406	0	0.0	803
N334S	15	405	-0.1006	656	3	2.31	661
N394H	15	406	0.1454	245	2	1.54	715
T415I	15	407	0.0064	512	4	3.08	610
A363S	15	408	0.0172	495	2	1.54	713
T500A	15	409	-0.1877	695	6	4.62	517
V503G	15	410	-0.0557	606	23	17.69	238
P463L	15	411	-0.1197	663	4	3.08	608
W436C	15	412	-0.1338	670	13	10.0	339
G404C	15	413	-0.2079	700	40	30.77	139
W353C	15	414	-0.3289	723	41	31.54	134
G447C	14	415	-0.8992	778	45	34.62	112
H519R	14	416	-0.0117	544	0	0.0	802
T470S	14	417	0.0243	478	8	6.15	425
R466K	14	418	0.1568	222	8	6.15	438
N501K	14	419	-1.6479	804	20	15.38	263
N460T	14	420	0.0007	527	4	3.08	604
S443C	14	421	-0.6079	758	34	26.15	176
F342I	14	422	-0.1858	693	30	23.08	188
L461I	14	423	0.3095	114	10	7.69	395
I402L	14	424	0.1501	234	37	28.46	157
L518V	14	425	0.0105	508	4	3.08	596
Q498K	14	426	-2.2222	822	7	5.38	471
K462R	14	427	0.1207	279	3	2.31	631
L518P	13	428	0.0395	448	15	11.54	330
G404S	13	429	-0.0999	655	16	12.31	319
N360K	13	430	0.0492	419	0	0.0	808
N360Y	13	431	0.0231	482	1	0.77	781
L441P	13	432	0.1585	218	37	28.46	156
P384R	13	433	0.1463	241	18	13.85	287
K458T	13	434	0.0811	358	10	7.69	393
D428A	13	435	-0.0473	592	5	3.85	545
T333K	13	436	0.1752	196	0	0.0	796
T385A	12	437	-0.0934	649	5	3.85	559
A344D	12	438	-0.0192	554	4	3.08	573
A352D	12	439	0.1946	176	37	28.46	158
P337R	12	440	0.0349	461	3	2.31	657
T333A	12	441	-0.0255	561	0	0.0	849
Y508C	12	442	0.9074	9	32	24.62	183
Y505F	12	443	0.1716	203	14	10.77	335
S438A	12	444	0.0295	475	0	0.0	851
T478P	12	445	-0.0308	567	11	8.46	377
E465A	12	446	0.2730	122	3	2.31	664
S459P	11	447	0.0949	326	22	16.92	248
I434L	11	448	0.1508	233	2	1.54	679
Y453H	11	449	0.3624	92	10	7.69	383
D428H	11	450	-0.0058	536	1	0.77	783
D364N	11	451	-0.1862	694	0	0.0	832
V524I	11	452	0.0486	422	1	0.77	775
V395A	11	453	0.0019	525	2	1.54	681
P507A	11	454	0.3306	104	26	20.0	216
N394I	11	455	0.0976	319	3	2.31	643
E471K	11	456	0.2027	168	7	5.38	458
W353L	11	457	-1.3819	799	45	34.62	113
R357I	11	458	0.0972	320	5	3.85	531
F392S	11	459	0.0039	521	15	11.54	329
A363P	10	460	0.1829	189	13	10.0	343
D420G	10	461	0.4079	81	44	33.85	120
D420A	10	462	0.4484	65	16	12.31	314
A520E	10	463	0.1656	210	3	2.31	634
C379G	10	464	-2.7246	837	85	65.38	23
A348P	10	465	0.6286	30	1	0.77	778
G496R	10	466	-1.3052	797	36	27.69	166
D389A	10	467	-0.0759	637	1	0.77	779
K462T	10	468	0.3344	102	5	3.85	532
V350A	10	469	0.5792	37	97	74.62	12
Y505C	10	470	-2.1547	818	78	60.0	40
S469L	10	471	-0.1155	662	2	1.54	678
R457K	10	472	0.9654	6	24	18.46	234
S349A	10	473	-0.1154	661	4	3.08	597
F338Y	10	474	-0.0443	585	12	9.23	359
V350I	10	475	1.2105	1	16	12.31	305
C379F	10	476	0.2834	118	23	17.69	244
V503D	10	477	0.3650	91	9	6.92	412
G504C	10	478	-0.1310	666	37	28.46	153
K356T	10	479	0.0913	333	7	5.38	464
Y449F	9	480	-0.3866	732	13	10.0	340
G502D	9	481	-3.1242	847	35	26.92	172
N394T	9	482	0.0359	457	3	2.31	636
E516G	9	483	0.0533	413	8	6.15	443
N448K	9	484	-0.3576	727	22	16.92	246
D389H	9	485	0.0468	426	0	0.0	827
V483G	9	486	0.3240	110	28	21.54	206
G476V	9	487	-1.0920	789	45	34.62	117
L513V	9	488	-0.1704	687	23	17.69	241
Q498L	9	489	-0.8396	776	15	11.54	321
D405H	9	490	0.1833	187	9	6.92	409
K424R	9	491	0.0462	428	31	23.85	187
T500I	9	492	-1.3079	798	4	3.08	598
L517V	9	493	0.0154	499	0	0.0	826
A352G	9	494	0.2458	138	13	10.0	347
N394Y	9	495	0.1639	213	6	4.62	488
K462E	9	496	0.2143	156	7	5.38	474
P384T	9	497	0.1514	231	19	14.62	271
G413A	8	498	0.0447	433	2	1.54	677
A397T	8	499	0.0594	404	6	4.62	485
A363T	8	500	0.0776	367	3	2.31	638
L441V	8	501	0.0545	412	2	1.54	682
Y365S	8	502	0.0071	511	12	9.23	368
I410T	8	503	-2.8866	842	82	63.08	30
P463H	8	504	-0.0234	557	2	1.54	668
L518Q	8	505	0.0718	376	4	3.08	600
N360S	8	506	-0.0269	563	3	2.31	647
S399A	8	507	0.1296	261	3	2.31	649
R403T	8	508	-0.1772	690	59	45.38	84
K444E	8	509	0.3330	103	17	13.08	302
S366T	8	510	0.0782	365	0	0.0	823
Q409L	8	511	0.1189	284	61	46.92	79
Q474K	8	512	0.1404	249	5	3.85	533
N439I	8	513	0.2157	154	12	9.23	367
S383F	8	514	0.1835	186	10	7.69	400
S366A	8	515	0.0314	469	0	0.0	822
S443Y	8	516	-0.8372	774	50	38.46	108
S443T	8	517	-0.2496	709	3	2.31	650
N388S	8	518	0.0564	408	1	0.77	793
Q506K	8	519	-0.7802	769	58	44.62	85
N448Y	8	520	0.1252	271	29	22.31	201
N360D	8	521	-0.0294	566	6	4.62	491
Q409K	8	522	0.3953	85	26	20.0	224
N450Y	8	523	0.3405	97	6	4.62	493
G404V	8	524	-0.9610	782	59	45.38	83
I468F	8	525	0.0456	430	0	0.0	820
F490C	8	526	0.5074	52	28	21.54	203
F515L	7	527	-0.0985	652	2	1.54	669
V395L	7	528	0.1254	270	0	0.0	836
P479T	7	529	0.5761	38	2	1.54	670
A372E	7	530	-0.0188	553	1	0.77	773
L425V	7	531	0.1721	201	19	14.62	273
V445G	7	532	-0.2554	712	20	15.38	260
G504A	7	533	0.2433	140	13	10.0	356
L455M	7	534	0.8557	12	6	4.62	486
T393N	7	535	0.0451	431	2	1.54	685
L517P	7	536	0.1190	283	4	3.08	607
N354I	7	537	0.0556	410	6	4.62	482
E406A	7	538	0.6043	33	30	23.08	191
Q414E	7	539	0.0085	509	18	13.85	281
G404R	6	540	-0.3900	734	33	25.38	177
F374Y	6	541	-0.0356	574	8	6.15	437
N481T	6	542	0.0677	387	2	1.54	712
R403S	6	543	-0.3648	730	76	58.46	45
R357T	6	544	0.0719	375	5	3.85	560
R509S	6	545	-1.5776	803	81	62.31	35
A344G	6	546	-0.0658	621	4	3.08	609
D405A	6	547	0.2097	163	7	5.38	476
C379S	6	548	-0.3001	720	72	55.38	54
G339A	6	549	-0.0112	543	2	1.54	711
Y495H	6	550	-0.2106	702	44	33.85	121
V395G	6	551	0.1808	190	29	22.31	202
P491L	6	552	-2.1840	819	75	57.69	47
V367D	6	553	0.1064	305	5	3.85	537
F497S	6	554	-2.9867	844	83	63.85	27
V407L	6	555	0.1467	240	10	7.69	399
I358L	6	556	0.1032	309	1	0.77	749
G416R	6	557	-1.9903	814	67	51.54	68
H519N	6	558	0.0207	486	1	0.77	746
K458Q	6	559	0.2349	148	6	4.62	516
Y453S	6	560	0.7893	15	67	51.54	67
K417Q	6	561	-0.1887	696	36	27.69	160
N450S	6	562	0.0456	429	10	7.69	384
Q414P	6	563	-0.1380	674	39	30.0	141
G447A	6	564	-0.5726	755	10	7.69	398
G502S	6	565	-1.3865	800	28	21.54	204
D428V	5	566	0.1068	304	2	1.54	683
Q506L	5	567	-0.4863	744	53	40.77	94
T500N	5	568	-0.7391	766	5	3.85	538
N370T	5	569	-0.0314	569	2	1.54	706
D389V	5	570	-0.0063	537	1	0.77	742
D389Y	5	571	0.2515	135	0	0.0	852
G504R	5	572	0.2727	123	26	20.0	217
G502C	5	573	-1.9885	813	55	42.31	91
N334T	5	574	-0.0739	636	1	0.77	741
L452V	5	575	0.3268	108	16	12.31	313
F392V	5	576	0.0044	520	6	4.62	506
T393A	5	577	-0.0705	629	2	1.54	704
N450I	5	578	0.4476	66	5	3.85	539
A522E	5	579	0.2085	165	3	2.31	633
R403G	5	580	-0.8237	773	72	55.38	53
R509K	5	581	-0.1553	681	51	39.23	104
A522G	5	582	0.1605	216	5	3.85	540
N437S	5	583	0.0417	443	15	11.54	325
S366P	5	584	0.0828	353	30	23.08	189
Q409E	5	585	0.1641	211	38	29.23	149
P463A	5	586	0.0760	371	5	3.85	541
I410L	5	587	0.9071	10	17	13.08	300
D364Y	5	588	-0.0456	589	5	3.85	542
A475P	5	589	-0.5698	754	31	23.85	186
I402T	5	590	0.7805	17	81	62.31	32
Y421C	5	591	0.1011	312	52	40.0	101
G447D	4	592	-0.8386	775	38	29.23	150
S383A	4	593	-0.0140	547	5	3.85	543
Y473C	4	594	-1.2423	795	55	42.31	92
K424N	4	595	0.0888	343	57	43.85	87
K386R	4	596	0.0335	463	5	3.85	530
C525F	4	597	0.2251	152	3	2.31	641
T345I	4	598	-0.0650	619	3	2.31	639
V510A	4	599	0.2575	132	25	19.23	229
K378E	4	600	0.1282	265	21	16.15	254
S438T	4	601	0.0026	524	0	0.0	853
Y421H	4	602	0.2031	167	32	24.62	184
G381E	4	603	-0.0721	631	11	8.46	374
Q506H	4	604	0.0489	421	30	23.08	190
F486V	4	605	0.3537	93	74	56.92	49
L441H	4	606	0.0190	490	1	0.77	757
N487D	4	607	-0.7586	768	53	40.77	95
P412L	4	608	-0.1990	697	6	4.62	499
T393S	4	609	0.0199	488	1	0.77	758
V341F	4	610	-0.0357	575	2	1.54	702
L492I	4	611	0.6538	27	16	12.31	318
V367G	4	612	-0.0493	597	4	3.08	574
N440H	4	613	0.3259	109	1	0.77	761
N334H	4	614	-0.0648	618	0	0.0	838
T430A	4	615	-0.0444	586	2	1.54	699
Y365C	4	616	-0.0129	546	33	25.38	179
R457M	4	617	0.0897	340	60	46.15	81
G404A	4	618	-0.1138	660	15	11.54	326
S459A	4	619	0.0427	441	0	0.0	843
S366Y	4	620	0.2410	143	1	0.77	765
D442E	4	621	-0.4202	736	45	34.62	116
L455W	4	622	0.1100	298	13	10.0	351
V362A	3	623	-0.0591	610	1	0.77	766
A475D	3	624	-1.2605	796	34	26.15	174
L368P	3	625	-0.2121	703	58	44.62	86
Y508F	3	626	0.3939	86	0	0.0	841
W353S	3	627	-0.2453	708	53	40.77	99
P507T	3	628	-2.7888	838	77	59.23	43
G502V	3	629	-3.0008	845	53	40.77	98
N370Y	3	630	0.2105	161	0	0.0	819
F486Y	3	631	-0.0522	598	13	10.0	352
F374I	3	632	-0.0665	622	7	5.38	446
G482C	3	633	0.4500	64	4	3.08	568
G485C	3	634	0.0933	328	21	16.15	252
R357G	3	635	-0.1639	683	9	6.92	421
G496D	3	636	-1.1076	790	73	56.15	52
F374S	3	637	-0.1321	668	17	13.08	292
F497Y	3	638	0.8793	11	67	51.54	66
G476R	3	639	-0.4374	739	21	16.15	251
C488F	3	640	-2.1905	820	76	58.46	46
G496C	3	641	-0.5235	748	37	28.46	159
N370I	3	642	0.0682	386	4	3.08	576
Y489C	3	643	-2.0570	817	68	52.31	60
F347L	3	644	-0.4723	742	4	3.08	578
S359C	3	645	0.0930	329	5	3.85	546
Y380H	3	646	-0.1581	682	8	6.15	436
N343Y	3	647	-0.0548	603	1	0.77	794
Q474L	3	648	-0.0727	633	6	4.62	489
T415P	3	649	0.0471	425	7	5.38	454
N394K	3	650	0.1222	277	3	2.31	652
V511A	3	651	0.2372	146	36	27.69	162
S514A	3	652	0.0346	462	2	1.54	697
E516V	3	653	0.0321	466	2	1.54	696
V407A	3	654	-0.0480	594	36	27.69	164
Q409H	3	655	0.0960	325	19	14.62	272
L518R	3	656	0.1421	247	3	2.31	658
P412Q	3	657	-0.4334	738	14	10.77	338
Y453C	3	658	0.6284	31	68	52.31	61
Y449C	3	659	-1.0458	787	56	43.08	90
L390I	3	660	0.0032	522	2	1.54	694
N448I	3	661	-0.2899	716	45	34.62	118
L335W	3	662	0.1639	214	0	0.0	806
V524F	3	663	0.0906	337	0	0.0	809
F429L	3	664	-0.1489	678	12	9.23	366
N439T	3	665	-0.5441	751	10	7.69	402
V433L	3	666	-0.4384	740	16	12.31	308
N439D	3	667	-0.4260	737	30	23.08	198
S438F	3	668	-1.1859	792	93	71.54	13
A435V	3	669	-0.0287	564	2	1.54	692
I468L	3	670	0.0766	369	3	2.31	662
L461F	3	671	0.0439	438	3	2.31	655
W353R	3	672	-0.7866	770	120	92.31	5
V382M	3	673	0.0153	500	2	1.54	691
T385S	3	674	-0.0532	599	4	3.08	584
L387F	3	675	0.0072	510	5	3.85	549
R509I	3	676	-1.7426	808	87	66.92	19
I468S	3	677	0.1498	236	7	5.38	452
A411D	2	678	0.4007	83	37	28.46	154
T470P	2	679	-0.1679	684	20	15.38	268
D420H	2	680	0.5080	51	12	9.23	362
P521A	2	681	0.0824	355	1	0.77	754
Y365H	2	682	0.0142	501	2	1.54	690
F377S	2	683	-0.2509	710	34	26.15	175
Q506R	2	684	-0.9634	783	28	21.54	205
F497I	2	685	-2.3234	825	60	46.15	80
R454G	2	686	-2.9796	843	81	62.31	33
R454K	2	687	0.9525	7	30	23.08	196
P499A	2	688	-0.2916	717	12	9.23	364
G476C	2	689	-0.9466	781	44	33.85	119
A411P	2	690	-2.3752	828	86	66.15	21
F338I	2	691	-0.0399	578	9	6.92	408
A411G	2	692	1.0159	2	30	23.08	192
F456Y	2	693	-0.8053	771	35	26.92	167
I358F	2	694	0.1513	232	0	0.0	810
T500P	2	695	-0.6046	757	13	10.0	353
I410S	2	696	-3.2485	850	88	67.69	17
T333S	2	697	-0.0440	584	0	0.0	805
W436R	2	698	-0.2735	714	17	13.08	298
Y505D	2	699	-3.2790	852	62	47.69	75
I434K	2	700	-0.9279	780	114	87.69	9
C379R	2	701	-2.3877	829	74	56.92	48
G502A	2	702	-1.8825	811	27	20.77	214
R355S	2	703	-0.1686	685	20	15.38	267
V382G	2	704	-0.0873	643	26	20.0	226
S383Y	2	705	0.1874	183	8	6.15	428
Y473D	2	706	-2.1943	821	64	49.23	72
N334I	2	707	-0.1352	672	0	0.0	798
K444Q	2	708	0.1935	177	10	7.69	387
V445D	2	709	-0.0686	626	13	10.0	354
P426A	2	710	0.1105	295	7	5.38	477
R357S	2	711	-0.0085	540	6	4.62	500
D420E	2	712	0.2599	130	35	26.92	171
D420V	2	713	0.9813	4	9	6.92	406
I410M	2	714	0.4619	61	41	31.54	132
N343K	2	715	-0.1348	671	3	2.31	659
S469P	2	716	-1.2212	794	82	63.08	29
V401A	2	717	0.2607	129	26	20.0	225
F392L	2	718	0.0054	515	4	3.08	586
G482A	2	719	0.1190	282	4	3.08	587
S373T	2	720	0.0047	517	0	0.0	807
A372G	2	721	0.0016	526	7	5.38	461
F342Y	2	722	-0.3964	735	22	16.92	245
N481H	2	723	0.0301	471	0	0.0	812
F342V	2	724	-0.1811	692	45	34.62	115
F347V	2	725	-0.3881	733	4	3.08	615
N487S	2	726	-1.0698	788	30	23.08	199
C480S	2	727	-2.3398	826	67	51.54	64
I468M	2	728	0.0963	322	2	1.54	689
P491R	2	729	-3.1152	846	104	80.0	11
Y369S	2	730	-0.0452	588	23	17.69	239
E465Q	2	731	0.0664	390	4	3.08	591
E465V	2	732	0.8400	13	10	7.69	401
P507Q	2	733	-3.2439	849	84	64.62	26
F464Y	2	734	0.0003	528	11	8.46	373
K458M	2	735	0.3098	113	6	4.62	480
N460H	2	736	0.2132	157	0	0.0	797
Q474P	2	737	0.4341	70	27	20.77	215
D405V	2	738	0.0193	489	9	6.92	420
T478S	2	739	-0.0550	604	7	5.38	468
L517R	2	740	0.1407	248	2	1.54	688
E465K	2	741	0.0906	336	5	3.85	550
L492S	2	742	-2.0329	816	62	47.69	76
Y351C	1	743	0.1551	227	19	14.62	270
S349F	1	744	-0.7341	765	36	27.69	161
N343D	1	745	-0.1431	676	6	4.62	501
R509G	1	746	-2.8208	840	85	65.38	24
T333R	1	747	0.1567	223	0	0.0	813
C525R	1	748	0.1269	268	5	3.85	551
Y508N	1	749	0.1447	246	47	36.15	110
F342S	1	750	-0.4673	741	69	53.08	58
N343S	1	751	-0.2160	705	2	1.54	687
V510E	1	752	-2.7094	836	128	98.46	1
F338V	1	753	-0.1787	691	16	12.31	306
N334D	1	754	0.0389	450	5	3.85	553
R509T	1	755	-0.6143	759	82	63.08	31
L513P	1	756	-2.8153	839	127	97.69	2
V524A	1	757	0.0228	483	3	2.31	660
E516D	1	758	0.1054	306	5	3.85	554
A520G	1	759	-0.0898	647	7	5.38	472
Q506P	1	760	-3.5875	853	114	87.69	8
S514C	1	761	0.1385	252	5	3.85	555
F347S	1	762	-0.2991	719	18	13.85	279
F347C	1	763	-0.3268	722	21	16.15	253
P507L	1	764	-3.2345	848	79	60.77	38
V512L	1	765	-2.2618	824	68	52.31	62
V341D	1	766	-0.0882	646	26	20.0	223
V512A	1	767	0.2415	142	35	26.92	169
S349Y	1	768	-0.6869	764	41	31.54	136
F347Y	1	769	0.0474	424	9	6.92	417
N437Y	1	770	-1.8301	810	16	12.31	307
G502R	1	771	-3.2607	851	28	21.54	209
P412T	1	772	-0.1494	680	15	11.54	324
R457G	1	773	0.7071	23	83	63.85	28
V407F	1	774	-0.0851	642	16	12.31	311
L461V	1	775	0.2114	159	17	13.08	304
E406V	1	776	0.5073	53	30	23.08	194
L461H	1	777	-0.7456	767	70	53.85	57
E406K	1	778	-0.1044	657	33	25.38	178
L461P	1	779	-2.4869	833	86	66.15	20
K462N	1	780	0.1021	311	4	3.08	571
P463R	1	781	-0.0707	630	5	3.85	556
F464C	1	782	-0.0065	538	34	26.15	173
F400Y	1	783	0.6711	24	46	35.38	111
F400V	1	784	-2.5774	834	88	67.69	18
S399T	1	785	0.1462	242	2	1.54	686
A397E	1	786	-2.0250	815	73	56.15	50
R466T	1	787	0.0617	397	7	5.38	462
R466S	1	788	-0.0014	532	8	6.15	439
Y396C	1	789	0.2128	158	6	4.62	508
F456S	1	790	-1.7142	807	71	54.62	56
P412S	1	791	-0.3775	731	18	13.85	289
D467H	1	792	-0.2189	706	16	12.31	309
L452P	1	793	-0.3427	724	68	52.31	63
N437I	1	794	-1.2066	793	38	29.23	151
A435P	1	795	-2.4472	832	120	92.31	6
S438Y	1	796	-1.0407	786	42	32.31	128
I434R	1	797	-2.4305	830	80	61.54	36
V433A	1	798	-0.3556	726	53	40.77	97
V433F	1	799	-2.2566	823	92	70.77	14
G431V	1	800	-2.3621	827	124	95.38	3
N439S	1	801	-0.4971	746	8	6.15	440
C525W	1	802	0.3368	101	6	4.62	502
D442H	1	803	-0.9957	784	105	80.77	10
V445L	1	804	0.1072	301	5	3.85	557
P426T	1	805	0.0895	341	4	3.08	606
Y421N	1	806	0.1250	272	51	39.23	106
G447R	1	807	-0.6553	763	23	17.69	237
N448H	1	808	-0.2941	718	17	13.08	294
A419P	1	809	0.6407	29	78	60.0	41
Y451C	1	810	0.0109	506	60	46.15	82
D467N	1	811	-0.2518	711	30	23.08	197
D467Y	1	812	-0.4749	743	9	6.92	410
N501D	1	813	-1.6930	806	38	29.23	147
C488R	1	814	-1.7761	809	79	60.77	39
Y489N	1	815	-1.6805	805	65	50.0	71
Y489S	1	816	-2.4469	831	67	51.54	65
Y489F	1	817	-0.1409	675	26	20.0	220
L368V	1	818	-0.0875	645	6	4.62	515
L492F	1	819	0.7863	16	27	20.77	212
Y495N	1	820	-0.5771	756	79	60.77	37
Y495D	1	821	-1.9168	812	85	65.38	25
Y495S	1	822	-1.5082	802	81	62.31	34
Y365D	1	823	-0.0724	632	42	32.31	130
D364E	1	824	-0.0452	587	3	2.31	663
Y495C	1	825	-1.4773	801	122	93.85	4
A363D	1	826	-0.0902	648	8	6.15	426
Y495F	1	827	0.4188	77	18	13.85	290
C361F	1	828	0.1543	229	4	3.08	603
F497L	1	829	0.1132	291	62	47.69	74
N360I	1	830	-0.2063	699	1	0.77	777
Q498P	1	831	-2.8546	841	76	58.46	44
C488W	1	832	-2.6861	835	73	56.15	51
N487T	1	833	-0.8465	777	31	23.85	185
D467G	1	834	-0.5248	749	36	27.69	163
S477C	1	835	0.1588	217	7	5.38	450
D467V	1	836	-0.2386	707	15	11.54	332
D467E	1	837	-0.0732	635	16	12.31	317
T393P	1	838	0.1530	230	7	5.38	449
F392I	1	839	0.0433	439	6	4.62	509
C391F	1	840	0.1886	181	5	3.85	558
C391S	1	841	0.0686	383	4	3.08	593
S469T	1	842	0.1756	194	1	0.77	776
I472N	1	843	-0.3218	721	20	15.38	266
I472S	1	844	-0.5591	752	24	18.46	233
I472M	1	845	0.1933	178	6	4.62	487
S383P	1	846	0.1276	266	18	13.85	286
V382A	1	847	-0.1325	669	11	8.46	382
G381A	1	848	0.0318	467	8	6.15	424
C379Y	1	849	0.0553	411	26	20.0	219
A475G	1	850	0.1737	197	45	34.62	114
K378T	1	851	0.0979	317	21	16.15	256
K378Q	1	852	0.0792	363	19	14.62	277
G526R	1	853	0.1668	208	5	3.85	528

* BFE change: Binding free energy change for the S protein and human ACE2 complex induced by a single-site S protein RBD mutation. A negative BFE change weakens the binding between S protein and ACE2, while a positive BFE change strengthens the binding between S protein and ACE2, giving rise to a more infectious variant [1].

♦ This column shows the number of antibody and S protein complexes disrupted by a specific mutation. We consider an antibody and S protein complex to be disrupted if its binding affinity is reduced by more than 0.3 kcal/mol.

♠ This column shows the ratio of disrupted antibody and S protein complexes out of 106 known complexes [2].